RNA extraction method
At present, the Trizol method is commonly used to extract RNA from tissues or cells, and there are other methods such as: DNA extractor , RNA extractor, DNA extraction kit, RNA extraction kit and so on. The Trizol method is a new type of total RNA extraction reagent, containing guanidine isothiocyanate and other substances, which can quickly disrupt cells and inhibit the nuclease released by the cells. Although Trizol contains RNase inhibitors, 1ml of Trizol generally can only lyse up to 100mg of tissue. However, if the tissue is excessive, Trizol cannot completely infiltrate the tissue and cannot completely lyse the tissue. The RNase and other substances in the excess tissue will cause the degradation of RNA. This is a very important reason for RNA degradation. At the same time, because RNA is easy to degrade, masks are generally required during the experiment to avoid tissue contamination. At the same time, the RNase-free imported pipette tips, EP tubes and DEPC water are selec...